Titanium dioxide nanoparticles, found in everything from cosmetics and sunscreen to paint and vitamins, caused systemic genetic damage in mice, according to a comprehensive study conducted by researchers at UCLA's Jonsson Comprehensive Cancer Center.
Titanium dioxide (TiO2) nanoparticles induced single- and double-strand DNA breaks and caused chromosomal damage, as well as inflammation, all of which increase the risk of cancer.
The UCLA study is the first to show that the nanoparticles had such an effect, said senior study author Robert Schiestl, UCLA professor of pathology, radiation oncology and environmental health sciences and a Jonsson Cancer Center scientist.
Once in the body, the TiO2 nanoparticles accumulate in different organs because the body has no way to eliminate them. And because they are so small, they can go everywhere, even through cells, and may interfere with sub-cellular mechanisms.
The study appears this week in the journal Cancer Research.
In the past, these TiO2 nanoparticles have been considered non-toxic because they do not incite a chemical reaction. Rather, it is the surface interactions the nanoparticles have within their environment — in this case inside a mouse — that causes the genetic damage, Schiestl said. They wander throughout the body causing oxidative stress, which can lead to cell death.
Robert Schiestl
It is a novel mechanism of toxicity, a physicochemical reaction, that these particles cause, in comparison to regular chemical toxins, which are the usual subjects of toxicological research, Schiestl said.
"The novel principle is that titanium by itself is chemically inert. However, when the particles become progressively smaller, their surface, in turn, becomes progressively bigger, and in the interaction of this surface with the environment, oxidative stress is induced," Schiestl said. "This is the first comprehensive study of titanium dioxide nanoparticle–induced genotoxicity, possibly caused by a secondary mechanism associated with inflammation and/or oxidative stress. Given the growing use of these nanoparticles, these findings raise concern about potential health hazards associated with exposure."
The manufacture of TiO2 nanoparticles is a huge industry, Schiestl said, with production at about 2 million tons per year. In addition to paint, cosmetics, sunscreen and vitamins, the nanoparticles can be found in toothpaste, food colorants, nutritional supplements and hundreds of other personal care products.
"It could be that a certain portion of spontaneous cancers are due to this exposure," Schiestl said. "And some people could be more sensitive to nanoparticle exposure than others. I believe the toxicity of these nanoparticles has not been studied enough."
Schiestl said the nanoparticles cannot go through the skin, so he recommends using a lotion sunscreen. Spray-on sunscreens could potentially be inhaled and the nanoparticles can become lodged in the lungs, he said.
TiO2 nanoparticles
In the study, mice were exposed to the TiO2 nanoparticles in their drinking water and began showing genetic damage on the fifth day. The human equivalent is about 1.6 years of exposure to the nanoparticles in a manufacturing environment. However, Schiestl said, it's not clear if regular, everyday exposure in humans increases exponentially as continued contact with the nanoparticles occurs over time.
"These data suggest that we should be concerned about a potential risk of cancer or genetic disorders, especially for people occupationally exposed to high concentrations of titanium dioxide nanoparticles, and that it might be prudent to limit their ingestion through non-essential drug additives, food colors, etc.," the study states.
Next, Schiestl and his team will study exposure to the nanoparticles in mice that are deficient in DNA repair, to perhaps help find a way to predict which people might be particularly sensitive to them.
The study was funded by the National Institutes of Health.
UCLA's Jonsson Comprehensive Cancer Center has more than 240 researchers and clinicians engaged in disease research, prevention, detection, control, treatment and education. One of the nation's largest comprehensive cancer centers, the Jonsson Center is dedicated to promoting research and translating basic science into leading-edge clinical studies. In July 2009, the Jonsson Cancer Center was named among the top 12 cancer centers nationwide by U.S. News & World Report, a ranking it has held for 10 consecutive years.
By Kim Irwin
Contact: Kim Irwin, 310-206-2805, kirwin@mednet.ucla.edu
